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41.
Pre-proparathyroid hormone is the major protein synthesized in wheat-germ extracts in response to addition of an 8-15S fraction of parathyroid RNA. The accuracy of the translation of the mRNA from parathyroid tissue was examined by analysis of the carboxyl-terminal tryptic peptide and the amino-terminal amino acid of the protein, by analysis of the size distribution of the mRNA, and by translation of the mRNA in a second cell-free extract. When 8-15S RNA was fractionated on a sucrose gradient containing formamide, RNA that supported the synthesis of pre-proparathyroid hormone was present in a single symmetrical peak, suggesting that it was homogeneous. Analyses by paper chromatography and electrophoresis of the proline-containing tryptic peptides of pre-proparathyroid hormone indicate that they are identical with the corresponding proline-containing peptides of parathyroid hormone. Because the COOH-terminal tryptic peptide of parathyroid hormone contains proline, the data indicate that the COOH termini of pre-proparathyroid hormone and parathyroid hormone are identical. Methionine from initiator [35S]Met-tRNAfMet was rapidly incorporated into pre-proparathyroid hormone by the wheat-germ extract, and a single-step Edman degradation selectively removed almost all of the initiator [35S]methionine present in pre-proparathyroid hormone. Translation of the 8-15S RNA in a cell-free extract from Krebs-II ascites cells resulted in a protein that comigrated with pre-proparathyroid hormone on sodium dodecyl sulfate-acrylamide gel electrophoresis. These data support the conclusion that the wheat-germ system accurately translates the mRNA for parathyroid hormone, and they strengthen the contention that pre-proparathyroid hormone is the initial biosynthetic product. 相似文献
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Erich Jost 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1970,40(6):251-256
Zusammenfassung Bei Kreuzungen zwischen Culex pipiens-Populationen verschiedener geographischer Herkunft werden drei Kreuzungstypen festgestellt: normale Kreuzbarkeit, reduzierte Kreuzbarkeit und Inkompatibilität (Nichtkreuzbarkeit). Die drei Kreuzungstypen sind mit Hilfe der Embryonierungsrate, der Schlüpfrate und der entstehenden Nachkommenschaft gegeneinander abgrenzbar. Bei Inkompatibilität sind 99.9% der sich entwickelnden Embryonen letal und etwa 0.1% der Tiere schlüpfen und sind fertile, diploide Weibchen. Die Aktivierung des Eies erfolgt durch das Spermium. Es liegt induzierte, meiotische Parthenogenese vor. Die diploiden, parthenogenetischen Weibchen gehen aus einer Oocyte 2. Ordnung oder aus Teilungsprodukten einer Oocyte 2. Ordnung hervor. Bei den letalen, haploiden Embryonen beteiligt sich das Spermium nicht am Aufbau des Embryos.
Teil einer Dissertation der Math.-Nat. Fakultät der Universität Mainz
Danksagung: Herrn Prof. Dr. H. Laven danke ich für die Bereitstellung des Untersuchungsmaterials sowie für die Anregungen bei der Durchführung der Arbeit.
Angenommen durch H. Stubbe 相似文献
Genetic investigations on the incompatibility in the Culex pipiens complex
Summary In crosses between populations of the mosquito Culex pipiens of different geographical origin three crossing types have been found (1) crosses with normal offspring (2) crosses with reduced offspring and (3) crosses that show almost total incompatibility. In the case of incompatible crosses 99.9% of the developing embryos are lethal and only about 0.1% of the embryos hatch and develop into fertile diploid females. The origin of the exceptional diploid females has been investigated by means of the marker genes Kuf, r, w, var, y and ru. These females develop from a diploid oocyte or from a diploid nucleus that originated by fusion of the pronucleus and the last polar body. This has been concluded from the frequency of homozygous and heterozygous offspring from heterozygous females, the frequency of equational separation of different genes and the distribution of cross-over gametes in the exceptional females. Based on the genetical data it is argued that induced meiotic parthenogenesis takes place. The sperm does not play any part in the production of the diploid females and the lethal embryos. After the activation of the egg the sperm moves to the center of the egg but it does not succeed to fuse with the pronucleus. As a result the pronucleus starts to develop into a haploid embryo in about 99.9% and only in a few cases is the diploidy restored by a change in the meiotic process in the egg.
Teil einer Dissertation der Math.-Nat. Fakultät der Universität Mainz
Danksagung: Herrn Prof. Dr. H. Laven danke ich für die Bereitstellung des Untersuchungsmaterials sowie für die Anregungen bei der Durchführung der Arbeit.
Angenommen durch H. Stubbe 相似文献
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Winfried Häuser Petra Klose Jost Langhorst Babak Moradi Mario Steinbach Marcus Schiltenwolf Angela Busch 《Arthritis research & therapy》2010,12(3):R79
Introduction
The efficacy and the optimal type and volume of aerobic exercise (AE) in fibromyalgia syndrome (FMS) are not established. We therefore assessed the efficacy of different types and volumes of AE in FMS. 相似文献47.
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